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17906461_m The ankle sprain injury is the most frequently observed injury in the emergency room. Up to 40% of individuals with a history of ankle ligament injury have residual pain complaints. Chronic ankle pain often is a result of laxity of one of the ankle ligaments. A good understanding of the anatomy of the ankle ligaments is important in understanding diagnosis and treatment of these injuries. Prolotherapy, PRP Prolotherapy and Biocellular Prolotherapy have been shown to be effective treatment options.